Genomic analysis and identification of potential duplicate accessions in Burkina Faso cassava germplasm based on single nucleotide polymorphism

Monique Soro1, 2, 3, 4*, Justin S. Pita1, 2, Koussao Somé3, 4, 5, Daniel H. Otron1, 2, Edwige Yéo1, 2, J. Musembi Mutuku1, James B. Néya3, 4, Fidèle Tiendrébéogo1, 3, 4* and Daouda Koné2,6

Cassava adaptation to climate change and its resistance to diseases are essential prerequisites for achieving food security in sub-Saharan Africa. The accessions collected from farmers’ fields are very important be...

Detection of Cassava Mosaic Disease and Assessment of Selected Agronomic Traits of Cassava (Manihot esculenta)

Musa Decius Saffa 1,*, Alusaine Edward Samura 1 , Mohamed Alieu Bah 2,*, Angela Obiageli Eni 3 , Ezechiel Bionimian Tibiri 4 , Adama Sagnon 4, Fidèle Tiendrébéogo 3 , Justin Simon Pita 3 , Prince Emmanuel Norman 5 and Raymonda Adeline Bernardette Johnson 1

A study was conducted in Sierra Leone to identify cassava plants that are asymptomatic and symptomatic to cassava mosaic disease (CMD) and collect planting materials for field trial establishment; determine the prevale...

Cassava Mosaic Disease persistence: evidence from farmer adoption patterns of management practices in Benin

D` ewanou Kant David Ahoyaa,b, * , Ettien Antoine Adj´ ei c , Serge S` etondji Houedjissin b , J´ erˆ ome Anani Houngue b , Martine Zandjanakou-Tachin d , Jacob Afouda Yabi a , Eveline Marie Fulbert Windinmi Sawadogo-Compaoree , Modeste Kan Kouassi c , Fid` ele Tiendr´ eb´ eogo c , Ousmane Ndoye f , Justin Simon Pita c , Corneille Ahanhanzo b

Cassava Mosaic Disease (CMD) remains a major constraint to cassava production in Sub-Saharan Africa despite extensive promotion of management practices. This study investigates the adoption and intensity of adoption of C...

Cassava mosaic disease in Burkina Faso: epidemiological aspects and disease management perspectives

Seydou Sawadogo1,2, Fidèle Tiendrébéogo1,2,3*, Ezechiel B. Tibiri1,2, Pakyendou E. Name1,2, Florencia Djigma2 , Lassina Traoré2, Justin S. Pita3 and Angela O. Eni3

Cassava Mosaic Disease (CMD) is the main biotic constraint to cassava production in West Africa. In Burkina Faso, efforts are underway to manage it sustainably using classical surveillance, including field monitoring a...

Reinfection Dynamics of Disease-Free Cassava Plants in Three Agroecological Regions of Côte d’Ivoire

John Steven S. Seka 1,2,* Bekanvié S. M. Kouakou 1,2 Konan Evrard B. Dibi 3 , Justin S. Pita 1,2, Modeste K. Kouassi 1,2, William J. -L. Amoakon 2 , Mariam Combala 1,2, Daniel H. Otron 1,2 , Brice Sidoine Essis 3 , , Angela O. Eni 2, Nazaire K. Kouassi 1,2 and Fidèle Tiendrébéogo 2,*

Cassava mosaic disease (CMD) is caused by begomoviruses and can result in yield losses of up to 90% in susceptible varieties. Using disease-free planting material from in vitro cultures is one of the most effective way...

Assessment of cassava mosaic disease (CMD) intensity and whitefly (Bemisia tabaci) population in Cameroon based on field surveys

Joseph Aime Lauryn Bahoya1,4, Lewis Dopgima Levai1, Patrick Fogang Kenfack1,2, Ekwa Yawa Monono1 , Desmond Neba Ngwa1, Fidèle Tiendrebeogo5, François Tchoumbougnang4, Nicolas Niemenak3, Angela Obiageli Eni5, Justin Simon Pita5, & Oumar Doungous1

Cassava mosaic disease (CMD) remains a major constraint to cassava (Manihot esculenta Crantz) production in Cameroon. This study aimed to generate updated epidemiological data on CMD across the country. Surveys were cond...

Artificial intelligence tool for cassava viral diseases diagnosis using participatory surveillance in Burkina Faso

Seydou Sawadogo 1,2, Fidèle Tiendrebeogo 1,2,3*, Ezechiel B. Tibiri 1,2, Pakyendou E. Name 1,2, Florencia Djigma 2 , Lassina Traoré 2, Justin S. Pita 3 and Angela O. Eni 3

In the area of plant health, there has been little work using participatory approaches to control emerging infectious diseases such as cassava mosaic disease (CMD) and cassava brown streak disease (CBSD). These disease...

Removing recalcitrance to the micropropagation of ve farmer-preferred cassava varieties in Cô te d’Ivoire by supplementing culture medium with kinetin or thidiazuron

John Steven S. Seka1,2 *, Modeste K. Kouassi 1,2, Edwige F. Ye´ o3 , Flavie M. Saki2, Daniel H. Otron1,2, Fidèle Tiendre´ be´ ogo2 * , Angela Eni2, Nazaire K. Kouassi 1,2 and Justin S. Pita1,2

In vitro micropropagation is a rapid method of multiplying healthy planting material to control Cassava mosaic disease (CMD), one of a major constraint to cassava production in Africa. However, some cassava varieties h...

Genetic Characterization and Variability of Cassava (Manihot esculenta) Accessions Cultivated in Southwest and North Central Nigeria Using Agromorphological Markers

Isaac O. Abegunde 1,2,*, Oghenevwairhe P. Efekemo 1,3, Olabode Onile-Ere 1,3, Folashade Otitolaye 1,3 , Olusegun A. Oduwaye 4, Thomas O. Fabunmi 5, Emmanuel O. Idehen 1,4 , Justin S. Pita 6, Fidèle Tiendrébéogo 6 Titus Alicai 7 and Angela O. Eni 1,6,*

Understanding the genetic variability among Nigerian cassava accessions is a crucial pre- requisite for its improvement. The objective of this study was to estimate the genetic variability among 477 cassava accessions...

Enhancing Farmers’ Capacity for Sustainable Management of Cassava Mosaic Disease in Côte d’Ivoire

Ettien Antoine Adjéi 1,2,* , Kassoum Traoré 1,3, Eveline M. F. W. Sawadogo-Compaore 4 , Bekanvié S. M. Kouakou 2 , John Steven S. Séka 2 , Dèwanou Kant David Ahoya 5 , Kan Modeste Kouassi 2, Nazaire K. Kouassi 2 and Justin Simon Pita 2,*

Cassava Mosaic Disease (CMD) is a major constraint to cassava production in Côte d’Ivoire, causing significant yield and income losses for smallholder farmers. Despite its high prevalence, farmers’ knowledge and unders...

Emergence of begomoviruses and DNA satellites associated with weeds and intercrops: a potential threat to sustainable production of cassava in Cô te d’Ivoire

Aya Ange Nate´ Yoboue´ 1,2 *, Bekanvie´ S. M. Kouakou 1,2 , Justin S. Pita1,2 *, Boni N’Zue´ 3, William J.-L. Amoakon 2,4 , Kan Modeste Kouassi 1,2, Linda Patricia L. Vanie´ -Le´ abo1,2 , Nazaire K. Kouassi 1,2, Fatogoma Sorho1 and Michel Zouzou1

Cassava (Manihot esculenta Crantz) plays a signi cant role in the livelihoods of people in Africa, particularly in Côte d’Ivoire. However, its production is threatened by begomoviruses which cause huge yield losses. So...

Improvement of Nanopore sequencing provides access to high quality genomic data for multi-component CRESS-DNA plant viruses

Daniel H. Otron1,2,3, Denis Filloux4,5, Andy Brousse4, Murielle Hoareau2, Babbitha Fenelon2, Cécile Hoareau2 , Emmanuel Fernandez4,5, Fidèle Tiendrébéogo1, Jean-Michel Lett2, Justin S. Pita1,3, Philippe Roumagnac4,5 and Pierre Lefeuvre2,6*

Background: Faced with the recrudescence of viral CRESS-DNA plant diseases, the availability of efficient and cost-effective tools for routine diagnosis and genomic characterisation is vital. As these viruses possess cir...

Identification and Full-Genome Characterisation of Genomoviruses in Cassava Leaves Infected with Cassava Mosaic Disease

Olabode Onile-ere 1,2,*, Oluwagboadurami John 2, Oreoluwa Sonowo 2, Pakyendou Estel Name 3 Ezechiel Bionimian Tibiri 3 , Fidèle Tiendrébéogo 3,4 , Justin Pita 4, Solomon Oranusi 1 , and Angela O. Eni 2,4,*

This study identified and characterised three Genomoviruses during a circular DNA- enriched sequencing project aimed at assessing the evolution of Cassava mosaic bego- moviruses in Nigeria. Using a combination of rolli...

Improving the diagnosis of cassava mosaic begomoviruses using Oxford Nanopore Technology sequencing

Mariam Combala1,2, Ezechiel B. Tibiri1,3, Justin S. Pita1,2 , Angela O. Eni1, Seydou Sawadogo1,3, Pakyendou E. Name1,3, Saïdou Zongo1,3 , Poupouanou Kouhoumouri1,3, Adama Sagnon1,3, Oghenevwairhe P. Efekemo4 , Olabode Onile-ere4, Allen Oppong5, Cyrielle Ndougonna1 & Fidèle Tiendrébéogo1,3

Cassava mosaic disease (CMD), caused by begomoviruses such as African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV), poses a threat to food security in sub-Saharan Africa. Conventional PCR ass...

Towards sustainable management of cassava mosaic disease: The impact of awareness campaigns in Benin

D` ewanou Kant David Ahoyaa,b, * , Jacob Afouda Yabi a , Jerome Anani Houngue b Serge S` etondji Hou´ edjissin b , Martine Zandjanakou-Tachinc , Ettien Antoine Adjei d Eveline Marie Fulbert Windinmi Sawadogo-Compaoree , Justin Simon Pita d , Corneille Ahanhanzo b

Transboundary diseases, such as cassava mosaic disease (CMD), represent a significant risk to food security and the livelihoods of millions of households in sub-Saharan Africa. To address this issue, awareness campaigns...

Datasets on agromorphological characters and distribution of cassava (Manihot esculenta L.) accessions cultivated in South-West and North-Central regions of Nigeria

Isaac O. Abegunde a,b, ∗ , Oghenevwairhe P. Efekemo a,c , Olabode Onile-ere Folashade Otitolaye a,c , Emmanuel O. Idehen b,d, Angela O. Eni a,e, ∗ a,c

The data presented here are agronomic indicators of cassava accessions collected during epidemiological surveys of cassava farms across South-West and North-Central Nigeria in 2021-2022. Cassava accessions were obtai...

CONSULTATIVE AND AWARENESS RAISING MEETING ON THE RESPONSE TO EAST AFRICAN CASSAVA MOSAIC – UGANDA OUTBREAK IN SIERRA LEONE

On the 18 th of September 2024, a consultative and awareness raising meeting instigated by WAVE was held in Freetown, Sierra Leone at the Ministry of Agriculture conference room on the EACMV-Ug outbreak and its impl...

CASSAVA MOSAIC DISEASE AND ABUNDANCE OF ITS WHITEFLY VECTORIN NORTH-WESTERN DEMOCRATIC REPUBLIC OF CONGO

O. LIKITI, G,R. DIKO, O. MUBENGA 1 and G. MONDE

Cassava mosaic disease (CMD) is rampant in the Democratic Republic of Congo (DRC). The objective of this study was to assess the significance of CMD and abundance of its whitefly (Bemisia tabaci) vector, in the North-W...

Effect of Wolbachia as biocontrol agent on fecundity and survival of cassava whitefly in northwest Nigeria

Muhammad Dan Yaro Magaji, Ibrahim Umar Mohammed, Ibrahim Yusuf Jega and Abdulrahman Musa

Whitefly (Be mis ia tabaci Genn.) is known to vectored Cassava mosaic virus (CMV) and Cassava brown streak virus (CBSV), these viruses caused major diseases of cassava in Africa. An experiment was conducted to determin...

VIRUSES ASSOCIATED WITH CASSAVA MOSAIC DISEASE AND THEIR ALTERNATIVE HOSTS ALONG NIGERIA-CAMEROON BORDER

L.A. EZEJI 1, 2 , A.O. ADEDIJI 2,3 , C.K. NKERE 1 , O.C. OGBE 1 , J.T. ONYEKA 1 and G.I. ATIRI

Cassava mosaic disease (CMD) threatens cassava production across the African continent. Because CMD is widespread in sub-Saharan Africa (SSA), it is particularly important to monitor border regions to prevent the intro...

Occurrence of cassava mosaic begomoviruses in national cassava germplasm preserved in two agro- ecological zones of Ivory Coast

William J.-L. Amoakon1,2 | Aya Ange Naté Yoboué2,3 | Justin S. Pita2,3 |J. Musembi Mutuku2 | Boni N'Zué4 | Mariam Combala2,3 | Daniel H. Otron2,3 | Maïmouna Koné2,3 | Nazaire K. Kouassi2,3 | Raoul Sié1

Cassava production in Ivory Coast is hampered by cassava mosaic disease (CMD), which is caused by several begomovirus species. To increase cassava production and conserve genetic resources, the Centre National de R...

FIELD SURVEY OF CASSAVA MOSAIC DISEASE AND ITS WHITEFLY (Bemisia tabaci Genn.) VECTOR IN KEBBI AND KATSINA STATES, NIGERIA

Musa, A., Yusuf, I. J., Yunus, B. A., Muhammad, A. and Mohammed, I. U.

Demand for cassava roots and its products by man and industries is rising in Nigeria. However, cassava mosaic disease (CMD) is threatening cassava production in the country. This research was conducted to assess the curr...

Cassava mosaic disease and its whitefly vector in Cameroon: Incidence, severity and whitefly numbers from field surveys.

Oumar Doungous a, *, Boutou Masky a, Dopgima L. Levai a, Joseph A.L. Bahoya a, Emile Minyaka b, Jacques F. Mavoungou c, J. Musembi Mutuku d, Justin S. Pita d

Cassava plays a key role in the food security and economy of Cameroon, but its production is constrained by cassava mosaic disease (CMD). However, comprehensive surveys of CMD in Cameroon have been lacking. This study ai...

Sodium Toxicity: Should NaOH Be Substituted by KOH in Plant Tissue Culture?

Oumar Doungous 1 * , Jameel M. Al-Khayri 2 and Modeste Kan Kouassi 3

1 The Central and West African Virus Epidemiology (WAVE), Biotechnology Laboratory, Ekona Regional Research Centre, Institute of Agricultural Research for Development, Yaoundé, Cameroon, 2 Department of Agricultural B...

Evaluation of Ten Cassava Varieties for Resistance to Cassava Mosaic Disease in Burkina Faso

Monique Soro1,2, Koussao Somé3,4,*, Fidèle Tiendrébéogo2,3, Justin S. Pita1,5, Rahim Romba6, Bouma James Néya2,3, Daouda. Koné1,7

Abstract The whitefly-transmitted cassava mosaic disease (CMD) caused by cassava mosaic geminiviruses (CMGs) is the most important disease threatening the production of cassava (Manihot esculenta Crantz). Among the...

Epidemiological assessment of cassava mosaic disease in Burkina Faso

Monique Soro, Fidèle Tiendrébéogo, Justin S. Pita, Edwig T. Traoré, Koussao Somé, Ezechiel B. Tibiri, James B. Néya, J. Musembi Mutuku, Jacques Simporé, Daouda Koné

Surveys were conducted in 2016 and 2017 across the main cassava-growing regions of Burkina Faso to assess the status of cassava mosaic disease (CMD) and to determine the virus strains causing the disease, using fie...

South West and North Central Nigeria: Assessment of cassava mosaic disease and field status of African cassava mosaic virus and East African cassava mosaic virus

Angela O. Eni, Oghenevwairhe P. Efekemo, Olabode A. Onile-ere, Justin S. Pita

Cassava mosaic disease (CMD), caused by cassava mosaic begomoviruses (CMBs), is a major threat to cassava production in Nigeria. The predominant CMBs in Nigeria are African cassava mosaic virus (ACMV), East African cas...

Optimum Time for Harvesting Cassava Tubers to Reduce Losses Due to Cassava Brown Streak Disease in Northeastern DRC

Honoré Muhindo1, François Wembonyama2, Odette Yengele1, Médard Songbo1, Willy Tata-Hangy3, Sikirou Mouritala3, Justin Pita4 & Godefroid Monde

The present study aimed to determine the appropriate time to harvest cassava tuberous root which minimize the losses due to cassava brown streak disease (CBSD) in the region of Yangambi, DRC. To achieve the aim of thest...

Cassava mosaic disease (CMD) in Benin: Incidence, severity and its whitefly abundance from field surveys in 2020

Jerome Anani Houngue a, Serge Setondji Hou´edjissin a, Corneille Ahanhanzo a, **, Justin S. Pita b, M´elaine S. Ella Hound´enoukon c, Martine Zandjanakou-Tachin

Cassava mosaic disease (CMD) is the main threat to cassava (Manihot esculenta Crantz) production in Benin. This study was conducted to assess CMD incidence, disease severity, and adult whitefly (Bemisia tabaci) popu...

Using Thermotherapy And Meristem Tip Culture For Producing Virus-Free Cassava Planting Material From Six Varieties Cultivated In Côte d’Ivoire

E.F Yéo, M.K. Kouassi, J.S. Pita, N.K. Kouassi, D. Koné, S-P. A. N’guetta

Cassava cultivation in Côte d’Ivoire is constrained by cassava mosaic disease (CMD) caused by African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMV-CM). The disease is transmitted prin...

UNSUCCESSFUL CASSAVA BROWN STREAK DISEASE (CBSD)EVALUATION ATTEMPTS IN WESTERN DEMOCRATIC REPUBLIC OF CONGO AND IMPLICATIONS WITH CASSAVA ROOT NECROSIS DISEASE (CRND) ETIOLOGY

1Bakelana Zeyimo, 2Tata Hangy,1Tevo Ndomateso, 2Mahungu NzolaMeso, 3Monde Godefroid , 4Tshilenge Kanana, 4Lema Ki Munseki and 4Kalonji Mbuyi

Cassava brown streak disease (CBSD) is the second most important virus disease after Cassava mosaic disease (CMD), infecting cassava (ManihotesculetaCrantz) in Africa. The disease is caused by two distinct viruses, Cas...

ASSESSING THE SEVERITY AND THE INCIDENCE OF CASSAVA ROOT NECROSIS DISEASE (CRND) IN WESTERN DEMOCRATIC REPUBLIC OF CONGO

Bakelana Zeyimo1,6, Justin Pita6, Mwangu Kuhima3, Tevo Ndomateso1,6, Mayanza Kingolo1,6, Betezi Makuka1, Tuwizana Nsimba1, Mukendi Tshizembe2, Tshiamala Ngeleka2, Mahungu Nzola Meso4

Cassava is the staple food in the Democratic Republic of Congo (DRC) where both the roots and leaves are consumed. This crop is susceptible to several viral diseases, including Cassava Mosaic Disease(CMD) and Cassava B...

Occurrence of Cassava Mosaic Begomovirus New Species and Ageratum Leaf Curl Cameroon Virus on Pepper (Capsicum annuum L.) in Togo

Kodjovi Atassé Dansou-Kodjo1,2, Assion Sétu Mivedor1, Kossikouma Djodji Adjata1*, Jérôme Duclercq2, Yawovi Mawuena Dieudonné Gumedzoe1

Cassava mosaic disease caused by the whitefly-transmitted begomoviruses (family Geminiviridae) is a major threat to cassava (Manihot esculenta Crantz) production, which can be intercropped with other plant...

Farmer Knowledge of Cassava Mosaic Disease and Management Practices in Ogun State

Eni, Angela Obiageli, Efekemo, Oghenevwairhe Pretty, Onile-ere, Olabode Azeez

This study assessed CMD knowledge and management practices of farmers in Ogun state Nigeria during a farmers' training exercise. A total of 101 farmers (80 male and 21 females) participated in this study. Only a few fa...

FIRST REPORT AND PRELIMINARY EVALUATION OF CASSAVA ROOT NECROSIS IN ANGOLA

Bakelana Zeyimo1,7,8†, Laura M. Boykin5, Mahungu Nzola4,Mavila Ndofula3, Matondo Magole2, Nlandu Nduandele3, Lunfuankenda Makuati9 ,Tevo Ndomateso1,8, Monde Godefroid6,7, Justin Pita7, Lema ki Munseki8 and Tshilenge Kanana1,8

Cassava is a main staple food for 800 million people world-wide. Production is limited by pest and pathogens. The most devastating cassava viruses are Cassava Brown Streak Virus and Uganda Cassava Brown Streak Virus-...

Attempts to Identify Cassava Brown Streak Virus in Western Democratic Republic of Congo.

Bakelana Zeyimo

Root necrosis similar to those of the cassava brown streak disease (CBSD) were observed on cassava in western provinces of the Democratic Republic of Congo (DR.Congo) in the early 2000’s. However molecular laboratory di...

Attempts to Identify Cassava Brown Streak Virus in Western Democratic Republic of Congo

Abstract Root necrosis similar to those of the cassava brown streak disease (CBSD) were observed on cassava in western provinces of the Democratic Republic of Congo (DR.Congo) in the early 2000’s. However molecular labo...

FIRST REPORT AND PRELIMINARY EVALUATIONS OF CASSAVA BROWN STREAK-LIKE ROOT NECROSIS IN CONGO REPUBLIC

ABSTRACT: In the last 10 years, the Cassava Brown Streak Disease has spread across Africa from the east coast of Africa to central Africa. Similar root necrosis to cassava brown streak disease have also been identified...

Evaluation of resistance to cassava mosaic disease in selected African cassava cultivars using combined molecular and greenhouse grafting tools

Jerome Anani Hounguea, Martine Zandjanakou-Tachinb, Hermine Bille Ngallec, Justin S. Pitad, Gilles Habib Todjro Cacaïa, Sergine E. Ngatate, Joseph Martin Bellc, Corneille Ahanhanzoa.

Cassava mosaic disease (CMD) threatens cassava (Manihot esculenta) production in Africa. A total of 24 selected cultivars were screened against CMD using combined molecular and greenhouse grafting tools. Disease s...

Incidence of cassava mosaic disease and associated whitefly vectors in South West and North Central Nigeria: Data exploration

ABSTRACT: Cassava mosaic disease (CMD) is one of the most economically important viral diseases of cassava, an important staple food for over 800 million people in the tropics. Although several Cassava mosaic virus spe...

Survey of farmers’ knowledge of cassava mosaic disease and their preferences for cassava cultivars in three agro-ecological zones in Benin

Abstract Background: Cassava is an important crop in Africa that is widely cultivated for its starchy tuberous root, whichconstitutes a major source of dietary carbohydrates. Cassava mosaic disease (CMD) is the most dev...

Phenotypic Evaluation of Cassava (Manihot esculenta Crantz) Genotypes to Cassava Mosaic Virus by Mechanical Methods of Transmission

Aims: Cassava (Manihot esculenta Crantz), subsistence crop in sub-Saharan Africa was threatened by cassava mosaic virus that caused a devastated disease. This study aims to test thermotherapy as sanitation method in me...

Impact Of Thermotherapy And Chlorothalonil On Plantlets Production Of Some Genotypes Of Cassava (Manihot Esculenta Crantz) Produce In Benin

ABSTRACT: Cassava (Manihot esculenta) is a starchy root plant of great economic importance in sub-Saharan Africa and particularly in Benin. Its production is confronted to virus diseases which cause a considerable losses...

A ribodepletion and tagging protocol to multiplex samples for RNA‑seq based virus detection: application to the cassava virome

Daniel H. Otron1,2,3, Justin S. Pita1,3, Murielle Hoareau2, Fidèle Tiendrébéogo1, Jean‑Michel Lett2 and Pierre Lefeuvre2,4*

Background: Cassava (Manihot esculenta, Crantz), is a staple food and the main source of calories for many populations in Africa, but the plant is beset by several damaging viruses. So far, eight families of virus infect...